Journal of Musculoskeletal Trauma

Original Articles

Clinical Applications of Autologous Cultured Osteoblasts: Case Report: Seok Jung Kim, In Young Ok, Cheong Ho Chang, Young Sik Kim, Jae Deog Jang, Won Jong Bahk, Joo Hyung Lee; J Korean Fract Soc 2004;17(2):197-201. Published online April 30, 2004; DOI: https://doi.org/10.12671/jkfs.2004.17.2.197

Abstract PDF: We treated 3 cases of fracture and 1 case of avascular necrosis of femoral head using autologous cultured osteoblasts injection. The stromal cells from the bone marrow were cultured to differentiate to osteoblasts for 4 weeks. The fracture sites of each patients were right ulnar shaft, left radial shaft, and left 5th metatarsal base. All of the fractures showed callus formations after 1 week of osteoblasts injection to the fracture site. After 4 weeks, callus formations were progressed. Avascular necrosis of femoral head was bilateral and both were Ficat stage II. Core decompression and allograft impaction were performed to the left, and core decompression and autologous cultured osteoblasts injection percutaneously after 4 weeks of the decompression operation were done to the right femoral head. CT images of 1 year from the operations showed trabecular bone formation and well maintained femoral head contour of the right femur, but resorption of the grafted bone for the left.

Effect of Growth Hormone on Osteoblast and New Bone Formation: Hae Ryong Song, Young Jin Kang, Ki Churl Chang, Seong Chang Yeon, Ja Min Koo, Hyeon Hui Kim; J Korean Soc Fract 2003;16(3):423-431. Published online July 31, 2003; DOI: https://doi.org/10.12671/jksf.2003.16.3.423

Abstract PDF: PURPOSE
To evaluate effect of growth hormone on osteoblast and new bone formation.
MATERIALS AND METHODS
Bone defect of the tibia with preserved periosteum was made and fixed with external fixator. Intramuscular injection of growth hormone for 8 weeks in experimental group and saline in control group was performed. New bone formation at the bone defect in radiograph and bone mineral density (BMD) by quantitative computed tomography were evaluated at 8 weeks after surgery. Rat osteosarcom cells were cultured in both group to evaluate cell viability of osteoblast, alkaline phosphatase activity, and mRNA expression of osteocalcin by RT-PCR.
RESULTS
Experimental group showed more callus formation and higher BMD at the bone defect site and the distal tibia compared to control group and there was significant difference. Proliferation of osteoblast, alkaline phosphatase activity, mRNA of osteocalcin at 5 days after culture were significantly higher in experimental group than those in control group.
CONCLUSIONS
Growth hormone has positive effect on osteoblast and callus formation in vivo and vitro studies.

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